Zhang YiShanghai Institute of Applied Physics, CAS, China
Zhang Yi received his B.S. degree in Biochemistry from Lanzhou University in 1993 and Ph. D in Physics from Shanghai Institute of Nuclear Research, Chinese Academy of Sciences (CAS) in 2001. Then he worked as a postdoc at the Department of Chemistry, Northwestern University for two and a half years. He joined the Shanghai Institute of Applied Physics, CAS as an associate professor in 2004. He was a Humboldt Research Fellow in the Max Planck Institute for Polymer Research in 2008. He became a full professor of the Shanghai Institute of Applied Physics in 2010. His current research interests focus on scanning probe microscopy, nanoscale surface chemistry, self-assembly and self-organization of biomolecules and nanomaterials. He is the author of 6 book chapters and over 90 peer-reviewed papers.
Title：Improving PCR Amplification by Graphene Oxide
SymposiumB06 Medical application
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Abstract
A novel application of graphene oxide (GO) in molecular biology is reported. It was illustrated that GO can effectively improve the performance of polymerase chain reaction (PCR) through the enhancement of the yield, sensitivity, and specificity of PCR with certain concentrations in the PCR system. With optimized GO concentrations, an increase of at least 3 folds in PCR yield was achieved, and a remarkable improvement in sensitivity was demonstrated in both complicated human genome and simple plasmid PCR systems. Moreover, it was also demonstrated that GO-assisted PCR can successfully amplify trace amount of DNA templates disregarding the disturbance of large amount of exogenous DNA in the system. This ability has not been adequately demonstrated with other nanomaterials that are used as PCR additives. In addition, it was found that GO with certain concentrations effectively improved the performance of multiplex PCR by enhancing its specificity, sensitivity, and yields. The mechanism for the improvement of PCR performance with GO was analyzed. It was found that GO is likely to optimize the DNA amplification mainly through adsorbing single-stranded DNA template and primers.
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